| 摘要 |
PURPOSE:To provide an enzyme electrode which is stabilized for a long period of time, fast in responsiveness, and is capable of preventing diffusion of an obstacle material to an H2O2 detecting electrode, by a method wherein the H2O2 detecting electrode is formed on one surface of a porous film, and an enzyme is secured in the hole of the porous film and to said electrode side. CONSTITUTION:A thin layer such as Pt is formed as an H2O2 detecting electrode 2 on one surface of a porous film 1 such as polycarbonate. After, for example, a glucose oxidase aqueous solution is dripped and developed on the film 1 formed on the electrode 2, it is dried. Then, after an enzyme is cured in steam of a crosslinking agent such as glutalaldehyde, it is washed by water, and an enzyme electrode 9 is obtained such that the enzyme is, like a 3a, secured in a hole 4 in the film 1, and it is, like a 3b, secured to the surface of the electrode 2 on the film 1. The enzyme electrode 9 is held to a cylindrical holder body 12 through the medium of a packing 10 by a cap 11 in a manner to bring the Pt electrode 2 side into contact with an electrolyte 13 to obtain an enzyme electrode which is suitable for quantitative determination of glucose and the like in blood. |
