| 摘要 |
PURPOSE:To make electric current distribution uniform over the entire surface of a supporting surface and to obtain electrophoresis images having good linearity by insulating the surface to be measured in parallel with the electrophoresis direction of a support consisting of polyacrylamide or agarose gel by a material having affinity to said gel. CONSTITUTION:Hems of a specified width are provided on both sides of a glass plate 1 applied with a silane coupling treatment. Or spacers 5 are adhered by means of silane coupling materials 3 on both sides of a substrate 4. Such substrate is bound with a gel 2 prepd. by mixing an amphoteric electolyte with polyacrylamide or agarose gel to form a substrate 8 which is then placed on a cooling plate 13 of an electrophoresis device. A phosphoric acid soln. is put in a + electrode side and an NaOH soln. in a - electrode side, and after preliminary migration, a sample such as blood serum is put in a hole 7 opened beforehand on the gel 2 for electrophoresis. Electrophoresis images having good linearity are obtained by sealing the gel on the side surface of the substrate 1 with the hems or spacers 5 in the above-mentioned way. This device is suited for sepn. and analysis of colloidal particles such as the protein in the serum, more particularly clinical analysis of humor. |
