| 摘要 |
<p>The present invention provides expression of the S-antigen of HBV in eucaryotic host cells, especially mammalian cells, whereby post-translational processes sufficient for formation of S-antigen aggregates occur, under conditions of minimum risk of contamination. The S-antigen aggregates produced are highly antigenic, capable of eliciting protective immunity against HBV infection, hence suitable for incorporation into a vaccine. A significant contribution of the present invention is the use of the normal S-antigen promoter of HBV, either alone or in tandem with a second promoter. The invention further provides a vector for HBV core antigen expression using the HBV core antigen promoter. The use of HBV core antigen promoter permits construction of an expression vector independent of any preexisting promoter of the vector. The core antigen promoter is also very active and will be useful for the construction of vectors for expression of other proteins.</p> |
