| 摘要 |
PURPOSE:To analyze the antigens in a sample easily with high accuracy in dry type by combining a sheet for reaction having the 1st and 2nd antibody layers on a liquid impermeable substrate, and an acceptive sheet having a marked antigen acceptive layer and a diffusion speed control layer on a liquid impermeable substrate. CONSTITUTION:A sheet a for reaction (a) provided successively with the 2bd antibody layer 2 using hydrophilic colloid as matrix, a diffusion speed control layer 3 compounded suitably with a hydrophobic polymer and a hydrophilic polymer, and the 1st antibody layer 4 on a liquid impermeable substrate 1 of a plastic film, and an acceptive sheet (b) having an acceptive layer (F acceptive layer) 6 for a marked (isotope or the like) antigen and a diffusion speed control layer 7 on a liquid impermeable substrate 5 are prepared. A sample (serum or the like) and a constant amt. of liquid contg. the marked antigen are developed on the layer 4 of the sheet (a) and after this is subjected to incubation, an easy to cleave vessel 3 contg. a developing soln. 9 is placed between the layer 7 and the layer 4 of the sheet (b), and pressure is applied to develop the soln. 9 to the sheets (a), (b). After a prescribed time, the sheets (a), (b) are separated and the respective marked bodies are determined. In this way, the antigens in the sample are analyzed easily with good accuracy.
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