| 摘要 |
PURPOSE:To provide a lipase having the properties of TG lipase, MG lipase and esterase, and useful as a biochemical reagent, digestive enzyme, ester exchange catalyst, etc., by culturing bacteria belonging to Staphylococcus genus. CONSTITUTION:The novel lipase having the following characteristics and obtained from the culturing product of bacteria belonging to Staphylococcus genus, e.g. Staphylococcus aureus 226 strain. (a) Substrate specificity; easily decomposes tributyrin, tricaprin, trilaurin and monoolein: (b) optimum pH; 6.0 for triolein and tributyrin: (c) optimum temperature; 60 deg.C for triolein and 50 deg.C for tributyrin: (d) pH stability; stable at pH>=5 for triolein and tributyrin, and deactivated rapidly at pH <=4; (e) thermal denaturation; rapidly deactivated at >=60 deg.C and the deactivation is promoted by bivalent metal ions: etc. |
