| 摘要 |
Stabilized liquid enzyme and coenzyme compositions are prepared for use in biological diagnostic determination of glucose. In one embodiment a composition is prepared containing in admixture an aqueous vehicle, a nicotinamide-adenine dinucleotide coenzyme, a nucleotide, an enzyme including hexokinase and/or glucose-6-phosphate dehydrogenase and an organic solvent to stabilize the enzyme, coenzyme and nucleotide. The compositions have a pH of 6.0 to 8.5 and may contain a bacteriostat such as an azide compound. A preferred organic solvent is a polyol. In an alternative embodiment, a two reagent liquid enzyme and coenzyme composition is prepared wherein the enzyme is stabilized in one solution and the coenzyme and nucleotide stabilized in another solution, and the two solutions combined when used. In the enzyme-containing solution, an ammonia salt can be used as an alternative to the organic solvent to stabilize the enzyme. The compositions exhibit excellent shelf life and the container in which the compositions are stored can be repeatedly opened for use without any substantial degradation of the enzyme, coenzyme and nucleotide.
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