| 摘要 |
PCT No. PCT/JP97/00748 Sec. 371 Date Nov. 10, 1997 Sec. 102(e) Date Nov. 10, 1997 PCT Filed Mar. 10, 1997 PCT Pub. No. WO97/34006 PCT Pub. Date Sep. 18, 1997A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.
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