MICROBIAL ANTIBODIES IMMUNOASSAY

摘要

Immunoassay for determining the presence of microbial antibodies in mammalian serum which comprises the steps of: A. Providing a reaction mixture comprising a buffered aqueous medium; serum to be sampled; antigen derived from said microbes and antiantibody to the serum to be tested, said antigen or anti-antibody bearing a detectable label; B. Incubating said reaction mixture at from about 4 to 45.degree.C. for from about 24 to 2 hours at a pH of from about 6.5 to 8.5 to form an antigenantibody conjugate when said microbial antibody is present in said serum sample; and C. Determining the level of said label as a measure of the presence of said antigen-antibody conjugate; characterized in that the volume ratio of anti-antibody to serum sample is adjusted to a value sufficient to form an agglutinate but less than required to effectuate precipitation of the antigen-anti-body conjugate.