KINETIC ASSAY FOR ALPHA-AMYLASE

摘要

A kinetic assay is provided for measuring alpha-amylase content in aqueous solutions. The assay is based on the following reactions: I alph--1,4 linked glucan alpha-maltose II III IV The concentration of alpha-amylase is determined by measuring the rate of increase in absorbance associated with the production of NADH which is a measure of the activity of alpha-amylase. A novel reagent system is used with the assay and comprises: a polysaccharide having glucose molecules primarily connected through alpha 1,4 linkage maltose phoaphorylase (MP) Phosphate ions (PO4?) beta-nicotinamide-adenine dinucleotide (NAD) or betanicotinamide-adenine dinucleotide phosphate beta-D-phospnoglucomutase (Beta-PGM) Glucose-6-phosphate Dehydrogenase (G6PDH).