THYROXINE BINDING GLOBULIN ASSAY USING RADIOLABELED THYROXINE AND IMMOBILIZED ANTI-TBG ANTIBODIES

摘要

REAGENT AND METHOD FOR TBG ASSAY The concentration of thyroxine binding globulin (TBG) in a fluid sample can be determined via radioassay technique with an immunochemical composite comprising anti-TBG antibodies immobilized on a water-insoluble carrier. The composite is incubated with the sample in a quantity sufficient to assure complexation of substantially all TBG in the sample to the antibodies of the composite. The quantity of TBG completed is determined with radiolabeled thyroxine (T4) which complexes with at least a portion of the complexed TBG. By separating the labeled composite from the remaining labeled T4, and counting the bound (or unbound) T4, the concentration of TBG can be determined from a standard curve.