| 摘要 |
PURPOSE:To measure the substrate concentrations of enzyme or activity of enzyme rapidly and readily by forming the enzyme electrode through mixing of the coenzyme directly and chemically bound to high molecular carrier and current collectors and using this. CONSTITUTION:Nicotineamide Adenine Dinucleotide (NAD, coenzyme) is reacted with, e.g., succinic acid anhydride to introduce carboxylic groups and this is reacted with amno hexyl cellulose (high polymer carrier) to fix the NAD on the cellulose through amide binding. The above-mentioned coenzyme 2 having been directly chemically bound 4 to the high polymer carrier 6 is mixed with current collectors (e.g., graphite powder) 3 and this is press-molded to form the NAD fixed electrode, after which it is dipped in a solution containing enzyme (e.g., lactic acid dehydrogenase) 1 to prepare the NAD-enzyme bond, which is then treated with crosslinking reagent (glutaraldenyde, etc.) to fix the enzyme. The substrate concentration measured by using this. If this is used in the state where enzyme is not bound, the measurement of enzyme activity is possible. |
