METHODS FOR EXTRACTING AND PURIFYING KALLIDINOGENASE

摘要

A method for extracting and collecting kallidinogenase(EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45 to about 60.degree.C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.