发明名称 Method of testing for phospholipases using a composition containing a uniform dispersion of a phospholipid
摘要 A uniform dispersion of a phospholipid in an aqueous based, salt containing solution may be obtained by dissolving the phospholipid in an alcohol chosen from the group consisting of methanol, ethanol, propanol, and isopropyl alcohol, and adding the phospholipid-alcohol solution to the aqueous based solution. The uniform dispersion will remain, even if the alcohol is evaporated or otherwise removed from the aqueous solution. If lecithin or certain other phospholipids useful in testing for the presence or activity of phospholipase C or A enzymes are uniformly dispersed in this manner, the resulting aqueous dispersion may be solidified by the addition of a hardening agent, such as agar, and the so-solidified dispersion used in an improved testing procedure for such enzymes. The enzymes will cause areas of hydrolysis to occur when placed in wells cut in the solidified dispersion, the size of these areas indicating the amount of enzyme present.
申请公布号 US4140579(A) 申请公布日期 1979.02.20
申请号 US19770784059 申请日期 1977.04.04
申请人 MONCLA, BERNARD J. 发明人 MONCLA, BERNARD J.
分类号 C12Q1/44;(IPC1-7):G01N31/14 主分类号 C12Q1/44
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