Solid phase synthesis with base N alpha-protecting group cleavage

摘要

An improved solid phase peptide synthesis method is disclosed utilizing base labile N alpha -amino acid protecting groups. Such method provides a more efficient synthesis procedure in that it eliminates acid treatment thereby preventing loss of peptide from the support during each deprotection cycle and eliminates a substantial number of wash cycles in each step. A preferred base labile N alpha -amino protecting group is the 9-fluorenylmethyloxycarbonyl group (Fmoc) and a preferred base for deprotection is piperidine.