| 摘要 |
<p>1524916 Determination of aryl hydrocarbon hydroxylase MASSACHUSETTS INSTITUTE OF TECHNOLOGY 16 May 1977 [24 May 1976] 1918/78 Divided out of 1524915 Heading G1B [Also in Division C3] An enzymatically active supernatant, particularly useful in evaluating the mutagenesis of tissue cells cultured in vitro, is produced by the steps of homogenizing mammalian tissue e.g. rat liver, in an aqueous suspension medium, removing relatively large particles to produce a post-mitochondrial supernatant, and filtering the post-mitochondrial supernatant with a filter having a pore size sufficient to remove contaminating bacteria e.g. from 0.22 to 0.3 micron pore size. To the supernatant there may be added from 0.25 to 1.0 micromoles per millilitre of nicotinamide adenine dinucleotide, oxidized form (NADH). The aryl hydrocarbon hydroxylase activity (AHH) of the supernatant product is determined by incubation with benzopyrene followed by fluorimetric evaluation of the 3-hydroxy benzopyrene product. Examples demonstrate that the AHH activity is considerably increased by the bacterial filtration of the supernatant and the (optional) addition of NADH.</p> |
