Alkyl amines improve detection of components of formaldehyde-fixed biological samples

摘要

Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.

主权项

1. A reaction mixture comprising,
formaldehyde cross-linked biological sample; and a sufficient amount of an alkyl amine selected from the group consisting of ethylenediamine and propylamine to release at least a portion of a cross-linked component of the formaldehyde cross-linked biological sample.