| 摘要 |
The present invention relates to a device for carrying out light-sheet microscopy comprising: injecting means (203.2); an immersion chamber (205); guiding means (301 to 305) arranged to guide a light beam from the injecting means (203.2) and as far as into the immersion chamber (205) along a guiding optical path; shaping means (302, 303, 305) arranged to shape the light beam so as to give it a shape that is elongate along one spatial dimension so as to create a light sheet in a sheet plane located in the immersion chamber (205); and an objective (202). The shaping means (302, 303, 305) are located along the guiding optical path between the injecting means (203.2) and the immersion chamber (205), and are located between a first plane perpendicular to the optical axis (113) of the objective (202) and passing through the aperture (314) of the immersion chamber (205) and a second plane perpendicular to the optical axis (113) of the objective (202) and located beyond the distal end (312) of the objective (202) relative to the first plane (316). |
