| 摘要 |
1,175,697. 5<SP>1</SP>-Nucleotides. TAKEDA YAKUHIN KOGYO K.K. (Takeda Chemical Industries Ltd). 20 Dec., 1966 [25 Dec., 1965], No. 56872/66. Heading C2C. 5<SP>1</SP>-Nucleotides are obtained by culturing under aerobic conditions a micro-organism which belongs to the genus Corynebacterium, Brevibacterium, Pseudomonas or Arthrobacter and which is capable of assimilating hydrocarbons on. a culture medium comprising a carbon source consisting mainly of hydrocarbons containing not less than 10% (volume/volume) of a normal paraffin or paraffins having from 9 to 23 carbon atoms, suspending the sufficiently grown micro-organism" in an aqueous alkaline solution of a pH of substantially 7À8 to substantially 10À5 at 30‹ to 45‹ C. until the intracellular ribonucleic acid of the micro-organism is degraded into 5<SP>1</SP>-nucleotides which come out of the cells and are accumulated in the medium and recovering therefrom the thus accumulated 5<SP>1 </SP>- nucleotides. Specified micro-organism species are Corynebacterium hydrocarboclastus, Corynebacterium fascians, Brevibacterium alkanollyticum nov. sp., Brevibacterium leucinophagum, Pseudomonas-alkanolytica nov. sp., Pseudomonas aeruginosa and Arthrobacter simplex. The Brevibacterium alkanolyticum nov. sp. and Pseudomonas alkanolytica nov. sp. are stated to be novel species which have been isolated from soil and their microbial characteristics are described. The hydrocarbon source may contain, apart from normal C 9 -C 23 paraffins, other hydrocarbons, e.g. branched paraffins, olefins, cyclic paraffins; aromatic hydrocarbons or normal paraffins having up to 8 or not less than 24 carbon atoms. Other carbon sources, e.g. a carbohydrate such as glucose may also be present and the culture medium should also contain a nitrogen source. A small amount of inorganic salts may also be added to the medium. The incubation in the first stage is usually carried out at 25‹ to 40‹ C. and the grown micro-organisms may or may not be separated from the culture medium before subjecting to the second suspension stage. The grown micro-organisms may prior to suspension be subjected to a freezing and thawing process to degrade the envelopes of the cells or may be treated with chemical agents such as phenol, cresol or sodium desoxycholate or with a surface active agent or an organic solvent either before or during the alkaline suspension step. A compound providing polybasic anions, e.g. a phosphate, pyrophosphate, polyphosphate or arsenate may also be added to the alkaline medium. Examples are given in which 5<SP>1</SP>- adenylic, 5<SP>1</SP>-guanylic, 5<SP>1</SP>-cytidylic and 5<SP>1</SP>-uridylic are recovered from the alkaline suspension medium.
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