Cell line of renal sarcomatoid carcinoma in persons of Han nationality and preparation method thereof

摘要

Provided is a renal sarcomatoid cell line RCC09HYF, of which the deposit No. is CCTCC C201130, and the preparation method of the renal sarcomatoid cell line. The renal sarcomatoid cell line RCC09HYF can grow for a long period and be steadily passaged in vitro. By tumorigenic experiments using in-situ animal models in vitro it has been found that: the tumorigenesis is relatively fast inside animals and 3-4 weeks after tumor inoculation, the transplanted tumors fill the whole abdominal cavity, and dyscrasia appears in above 50% of nude mice; moreover, lung metastasis is present in a few individuals. The renal sarcomatoid cell line RCC09HYF can provide an effective and steady cell model for further study of the genesis and metastasis mechanism of renal sarcomatoid carcinoma in persons of Han nationality and for clinical prediction, diagnosis and treatment.

主权项

1. A method for generating a sarcomatoid renal cell carcinoma (sRCC) cell line from a Han Chinese patient comprising:
providing primary tumor tissue from a Han Chinese sRCC patient; immersing said primary tumor tissue in serum-free culture medium at about 4° C. for about 30 min; cutting said immersed primary tumor tissue into approximately 1-3 mm3 pieces; washing said primary tumor tissue pieces in serum-free culture medium; suspending said washed primary tumor tissue pieces in complete culture medium; seeding said suspended primary tumor tissue pieces into a culture vessel; and incubating said seeded primary tumor tissue pieces for 24 h at 37° C. under 5% CO2 and 95% humidity until at least some seeded primary tumor tissue pieces adhere to the culture vessel, wherein said adherent primary tumor tissue pieces are an sRCC culture.