Methods for collecting and using placenta cord blood stem cells

摘要

An innovative method of collecting cord blood stem cells from an isolated mammalian non-exsanguinated or partially exsanguinated placenta by placental perfusion is described and also an easy method for safe long duration cold storage of the placenta. Placental perfusion can include perfusing the isolated placenta with a pulsatile flow of perfusion solution, for example, using a pulsatile or peristaltic pump or device. The stem cells can then be isolated from the perfusate. Significantly increased amounts of CD133+ stem cells can be collected from the perfusate. The perfusion solution can include an anticoagulant. The isolated mammalian placenta need not be treated with an anticoagulant prior to perfusing. The isolated placenta can be free from an anticoagulant prior to perfusing.

主权项

1. A method for treating a mammal comprising a damaged tissue, comprising:
a) providing isolated cord blood stem cells by: i) performing machine perfusion of an isolated mammalian placenta comprising cord blood comprising cord blood stem cells, said performing machine perfusion being done with a pressure mediated pulsatile flow of a perfusion solution to produce a perfusate comprising cord blood comprising cord blood stem cells, wherein said isolated mammalian placenta is non-exsanguinated; ii) collecting the perfusate; and iii) isolating the cord blood stem cells from the perfusate to produce isolated cord blood stem cells; and b) introducing into a mammal comprising a damaged tissue a composition comprising a therapeutically effective amount of cells selected from the group consisting of the isolated cord blood stem cells and cells derived therefrom, whereby the damaged tissue is treated.