METHOD OF EUKARYOTIC EXPRESSION CLONING OF DISEASE ASSOCIATED MOLECULES

摘要

The present invention describes a method for identifying a target disease associated molecule (DAM) comprising: generating an expression library from DNA or RNA derived from a cell expressing the DAM; transfecting the library into a eukaryotic host cell; and screening the expression library with a screen comprising a binding partner to identify DNA clones expressing the target DAM. The library may produced by inserting the cDNA library into a virus based vector, such as a pox viral vector with a minimal viral genome.