A METHOD FOR PRODUCING STROMAL CELL-DERIVED FACTOR-1A AS AN ACTIVE SOLUBLE PROTEIN FORM

摘要

Disclosed is a method for the production of an active form of human stromal-derived factor (SDF-I a), featuring the renaturation of an inclusion body of proteins expressed in a large quantity in E. coli into an active form using a column. Also, an active, solubilized SDF- I a is provided. The protein refolding system is simpler and faster than conventional methods using dialysis or chaperones, thus having useful applications to other inclusion bodies.