| 摘要 |
The present invention provides a method for in-cell activity profiling of human receptor tyrosine kinases (RTK), comprising the following steps: - transferring plasmid vectors carrying wild-type human C-terminally V5/His-tagged RTKs or their disease-associated mutants into cell cultivation containers, - reconstituting the plasmid DNA and adding a transfection reagent, - adding cells to be transfected, - growing transfected cells to enable the RTK expression and spontaneous, ligand-independent dimerization and activation when in overexpressed state, - exposing the transfected cells to a compound to be tested and/or optionally to a cognate RTK ligand, - and performing a detection method. The invention further provides a selection of RTKs and their disease-associated mutants suitable for use in such method. ˙ |
