发明名称 Expression of granular starch hydrolyzing enzyme in trichoderma
摘要 The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.
申请公布号 US9382563(B2) 申请公布日期 2016.07.05
申请号 US201314097168 申请日期 2013.12.04
申请人 DANISCO US INC. 发明人 Baldwin Toby M.;Bower Benjamin S.;Dunn-Coleman Nigel;Lantz Suzanne E.;Pepsin Michael J.
分类号 C12N9/34;C12N1/20;C12N1/00;C12N15/00;C12P21/06;C07H21/04;C12P19/14;C12N1/14;C12N9/30;C12P19/20;C12P19/24;C12P19/02;C12P19/04 主分类号 C12N9/34
代理机构 Danisco US Inc. 代理人 Danisco US Inc.
主权项 1. A method for producing a granular starch hydrolyzing enzyme having glucoamylase activity (GSHE) in a filamentous fungal host cell comprising a) transforming a filamentous fungal host cell with a DNA construct comprising a promoter having transcriptional activity in the filamentous fungal host cell operably linked to a heterologous polynucleotide encoding the GSHE polypeptide of SEQ ID NO: 6, b) cultivating the transformed filamentous fungal host cell in a suitable culture medium to allow expression of said GSHE, and c) producing the GSHE.
地址 Palo Alto CA US