发明名称 |
Expression of granular starch hydrolyzing enzyme in trichoderma |
摘要 |
The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity. |
申请公布号 |
US9382563(B2) |
申请公布日期 |
2016.07.05 |
申请号 |
US201314097168 |
申请日期 |
2013.12.04 |
申请人 |
DANISCO US INC. |
发明人 |
Baldwin Toby M.;Bower Benjamin S.;Dunn-Coleman Nigel;Lantz Suzanne E.;Pepsin Michael J. |
分类号 |
C12N9/34;C12N1/20;C12N1/00;C12N15/00;C12P21/06;C07H21/04;C12P19/14;C12N1/14;C12N9/30;C12P19/20;C12P19/24;C12P19/02;C12P19/04 |
主分类号 |
C12N9/34 |
代理机构 |
Danisco US Inc. |
代理人 |
Danisco US Inc. |
主权项 |
1. A method for producing a granular starch hydrolyzing enzyme having glucoamylase activity (GSHE) in a filamentous fungal host cell comprising
a) transforming a filamentous fungal host cell with a DNA construct comprising a promoter having transcriptional activity in the filamentous fungal host cell operably linked to a heterologous polynucleotide encoding the GSHE polypeptide of SEQ ID NO: 6, b) cultivating the transformed filamentous fungal host cell in a suitable culture medium to allow expression of said GSHE, and c) producing the GSHE. |
地址 |
Palo Alto CA US |