| 摘要 |
<p>Aggregation is a major cause of the misbehavior of proteins. A system for modifying a protein to create a more stable variant is provided. The method involves identifying non- conserved hydrophobic amino acid residues on the surface of a protein, suitable for mutating to more hydrophilic residues (e. g., charged amino acids). Any number of residues on the surface may be chang ed to create a variant that is more soluble, resistant to aggregation, has a greater ability to re-fold, and/or is more stable under a variety of condit ions. The invention also provides GFP, streptavidin, and GST variants with a n increased theoretical net charge created by the inventive technology. Kits are also provided for carrying out such modifications on any protein of int erest.</p> |
