Beta-lactam antibiotic producing strains

摘要

The present invention relates to a method for the identification of one or more genes of a parent microbial strain capable of producing a ²-lactam compound and to mutant microbial strains in which the identified genes have been the functionally inactivated which results an at least 10% higher concentration of the total ²-lactam compounds in the culture medium compared to the parent microbial strain and/or an at least 10% higher concentration of the desired ²-lactam compound in the culture medium compared to the parent microbial strain; and/or an at least 10% improved yield (²-lactam / consumed sugar); and/or an at least 5% improved yield in desired ²-lactam compound compared to undesired/total beta-lactam compound; and/or an at least 10% decreased consumption of side-chain precursor