发明名称 |
TEMPLATE SWITCH-BASED METHODS FOR PRODUCING A PRODUCT NUCLEIC ACID |
摘要 |
Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (DNA), a polymerase, a template switch oligonucleotide, and dNTPs into a reaction mixture. The components are combined into the reaction mixture under conditions sufficient to produce a product nucleic acid that includes the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits. |
申请公布号 |
EP2912197(A4) |
申请公布日期 |
2016.07.13 |
申请号 |
EP20130848994 |
申请日期 |
2013.10.18 |
申请人 |
CLONTECH LABORATORIES, INC. |
发明人 |
BETTS, CRAIG;FARMER, ANDREW ALAN |
分类号 |
C12N15/10;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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地址 |
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