| 摘要 |
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Described is a method of identifying an essential gene which serves as an antibiotic target in a bacterium. Method includes steps of: generating an antibiotic resistant mutant of said bacterium by a method comprising a step of selecting for growth in presence of said antibiotic to produce an antibiotic resistant mutant clone (Abmutant); transforming Abmutant with: (i) one or more essential genes of said bacterium; and (ii) a transposon which insertionally inactivates bacterial DNA, to produce a pool of transposon mutants which are merodiploid for said one or more essential genes; growing bacteria from merodiploid pool in presence of different amounts of said antibiotic to produce two or more test cultures; and comparing distribution of transposon insertions between test cultures to identify a putative essential gene serving as a target of said antibiotic in said bacterium.EFFECT: invention makes it possible to identify target-candidates for antibiotics.12 cl, 2 dwg, 1 ex |
