PRODUCTION METHOD OF PSEUDOISLET

摘要

PROBLEM TO BE SOLVED: To provide methods of producing a practical pseudoislet suitable for medical transplantation, and the like by the high efficient induce differentiation of a pluripotent cell into a pancreatic islet progenitor cell or an islet cell with simple operations.SOLUTION: A production method of a pseudoislet comprises: seeding 1500-5000 cells/well of pluripotent cells in a cell culture well and producing a pluripotent cell aggregate with a given size; and culturing the aggregate in non-adhesive conditions in the cell culture well and differentiating it into a cell containing a pancreatic islet progenitor cell or an islet cell.SELECTED DRAWING: Figure 6