METHOD FOR SPECIFIC KNOCKOUT OF SWINE SLA-3 GENE USING CRISPR-CAS9 SPECIFICITY, AND SGRNA USED FOR SPECIFICALLY TARGETING SLA-3 GENE

摘要

Provided is a method for knockout of a swine SLA-3 gene using CRISPR-Cas9 specificity, and an sgRNA used for specifically targeting the SLA-3 gene. The target sequence of the sgRNA in the specifically targeted SLA-3 gene is located at the 5 exon coding regions, or the junction with the adjacent introns, of the N end of the SLA-3 gene.