COMPOSITIONS AND METHODS FOR ACTIVATING SILENT GENE CLUSTERS

摘要

The disclosure provides compositions and methods for producing natural products in microorganisms that are otherwise unexpressed, poorly expressed or poorly transcribed. In particular aspects, the disclosure provides compositions and methods for activating a silent gene or gene cluster with a bacteriophage and/or Streptomyces Antibiotic Regulatory Protein (SARP) transcription factor.

主权项

1. A method of activating one or more genes in a bacterial cell, the method comprising: infecting the bacterial cell with a bacteriophage, wherein the bacteriophage comprises a nucleotide sequence that encodes one or more bacterial transcription factors, and the bacterial cell expresses the one or more genes because of the expression of the one or more bacterial transcription factors, wherein the one or more genes are transcriptionally silent, poorly expressed, or poorly transcribed in a control bacterial cell that has not been infected with the bacteriophage comprising the nucleotide sequence encoding one or more transcription factors.