PHOSPHORAMIDITE DERIVATIVES, THEIR PREPARATION AND THE USE THEREOF IN THE INCORPORATION OF REPORTER GROUPS ON SYNTHETIC OLIGONUCLEOTIDES

摘要

2081320 9117169 PCTABS00008 Phosphoramidite derivatives of formulae (V) or (XII), wherein X is a reporter group and Y and Z are protecting groups. The reporter group X may comprise any hapten or other detectable moiety and examples include boitin, dinitrophenyl, dansyl and fluoresceinyl. There may be a linker arm, of variable length, between the reporter group and the rest of the molecule. Examples of the protecting groups, Y and Z, include 4,4'-dimethoxytrityl, trifluoroacetyl and fluorenylmethoxycarbonyl (Fmoc). The phosphoramidite derivatives are useful in the single or multiple labelling of synthetic oligonucleotides. Processes for the preparation of these phosphoramidite derivatives are also disclosed.