发明名称 |
Protein A-silica immunoadsorbent and process for its production |
摘要 |
An immunoadsorbent material for removing IgG and IgG-complexes from biological fluids is prepared by covalently binding protein A to a solid-phase silica matrix. It has been found that particularly stable, high-capacity immunoadsorbents are obtained by derivatizing the silica with amino and/or carboxyl groups, and reacting the protein A with a carbodiimide at a pH in the range from 3.5 to 4.5. Binding through free hydroxyl groups may be achieved with cyanogen halides at a pH in the range from 11.0 to 11.5. After acid washing (pH 2.0-2.5) to remove non-covalently bound protein A, the immunoadsorbent may be employed in a column for therapeutic treatment of various cancers and autoimmune disorders where IgG-complexes are implicated as suppressing factors in inhibiting a normal immune response.
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申请公布号 |
US4681870(A) |
申请公布日期 |
1987.07.21 |
申请号 |
US19850690781 |
申请日期 |
1985.01.11 |
申请人 |
IMRE CORPORATION |
发明人 |
BALINT, JR., JOSEPH P.;HARGREAVES, RICHARD E. |
分类号 |
C07K1/22;A61K39/00;A61K39/395;A61M1/36;A61P35/00;A61P37/00;B01J20/26;B01J20/32;C07K1/16;C07K17/14;G01N33/552;(IPC1-7):B01J20/22 |
主分类号 |
C07K1/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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