发明名称 Organotypic DRG-Peripheral Nerve Culture System
摘要 Techniques and systems are disclosed for a bioassay that is an in vitro mimic of peripheral nerve generation using the sensory neurons that innervate the peripheral nervous system. In some embodiments, the techniques may assist in detecting the bioactivity or potency of nerve grafts (e.g., processed, acellular human allografts) for fostering or supporting peripheral nerve regeneration. In various embodiments, techniques comprise affixing a harvested sensory neuron (e.g., a DRG) to a nerve graft segment to form a test construct; culturing the test construct in a medium; analyzing the test construct to indicate the amount of outgrowing peripheral nerve structure; and determining the potency of the nerve graft from a metric derived from the analysis. In some embodiments, techniques and materials may be used to test the effect of a varied test condition on peripheral nerve growth.
申请公布号 US2016349231(A1) 申请公布日期 2016.12.01
申请号 US201514724365 申请日期 2015.05.28
申请人 AxoGen, Inc. 发明人 DEISTER Curt
分类号 G01N33/483;G06T7/60;G06T7/00 主分类号 G01N33/483
代理机构 代理人
主权项 1. A method for conducting a bioassay of a nerve graft, the method comprising: affixing a dorsal root ganglion (DRG) to a first end of a nerve graft segment to form a test construct; culturing the test construct in a medium for a period of time to allow nerve outgrowth from the DRG into the nerve graft segment; performing an analysis of the test construct, wherein the analysis indicates an amount of an outgrowing peripheral nerve structure; and determining a potency of the nerve graft from a metric derived from the analysis.
地址 Alachua FL US