| 摘要 |
<p>1391028 Protein/enzyme complexes with macromolecular substances UNITED STATES ATOMIC ENERGY COMMISSION 11 May 1973 [6 June 1972] 22501/73 Headings C3H and C3P Biocompatible and biofunctional surfaces are prepared by radiation grafting a reactable compound comprising a hydrophilic hydrogel selected from polymers and copolymers on to an inert polymeric substrate and thereafter chemically bonding a biologically active molecule on to said reactable compound. The said hydrogel may be first chemically activated with, e.g. cyanogen bromide, thiosphosgene or carbodiimide, before being contacted with the biologically active molecule, e.g. human serum albumin, heparin, streptokinase or prostaglandin #-1. In another embodiment, the hydrogel is chemically activated, contacted with a solution of a second reactable compound which provides an intermediate chemical arm when bonded to the activated surface of the hydrogel, the second reactable compound is then activated and the biologically active molecule is bonded to the resulting activated surface. Alternatively, the hydrogel surface is contacted with a solution containing both a biologically active compound and a bifunctional coupling agent, e.g. glutaraldehyde, a diepoxide or diacid chloride. Examples are given in which silicone rubber films grafted with homopolymer of hydroxyethyl methacrylate, or hydroxyethyl methacrylate/N-vinyl pyrrolidone copolymer, are activated with cyanogen bromide and optionally treated with epsilon-amino caproic acid, or with the said acid followed by 1-cyclohexyl-3- [2-morpholinoethyl]-carbodiimide, before being contacted with albumin, heparin or streptokinase.</p> |
