A COMPOSITION COMPRISING AN IMMOBILISED, ACYLATED PEPTIDE

摘要

A novel generic coating procedure to improve the coating efficienncy of small synthetic peptide antigens in ELISA is described. Inthis invention the binding capacities of several peptides linked to various moieties were compared to their parent counterparts in ELISA. Elongation of an epitope-sequence by an acyl group of the formula: R1-A-R2-CO- wherein R1 is lower alkyl, arly, or aralkyl; R2 is a divalent radical derived from lower alkyl, arly, or aralkyl; and A is a sulfur containing functional group selected from -CO-S-, -S-, -SO-, -SO2- and -S-S- specifically increased the binding reactivity with the monoclonal antibody when adsorbed onto a solid support, preferably polystyrene wells. Elongation is preferably, N-teminally. Several moieties achieved an enormous reduction in peptide coat concentration for all tested peptides of net two to four orders of magnitude. Replacement of an Ata-extension (wherein R1 is methyl; R2 is methylene; and A is -CO-S-) by analogues (i.e. Bta, Atp, Btb) was possible without reducing their enhancing properties to a great extent. Furthermore, direct synthesis of the Ata-group to the N-terminal end of a peptide appears to be a simple and general strategy for effective adsorption of small peptides to polystyrene.