发明名称 |
IONIC STRENGTH-MEDIATED PH GRADIENT ION EXCHANGE CHROMATOGRAPHY PH |
摘要 |
The present invention provides methods for analyzing compositions of polypeptides such as antibodies by ionic strength-mediated pH gradient ion exchange chromatography. In some aspects, the methods use a combination of pH gradients and ionic strength gradients to separate the polypeptide from charge variants of the polypeptide. In some aspects, the methods use a stable ionic strength to optimize the pH gradient separation window to separate the polypeptide from charge variants. Such methods are useful for analyzing polypeptide, e.g. antibodies, with a pI greater than 9 or a pI less than 7. In some aspects, the invention provides a multiproduct method for the analysis of polypeptides of varying pI's. |
申请公布号 |
HK1217232(A1) |
申请公布日期 |
2016.12.30 |
申请号 |
HK20160105250 |
申请日期 |
2016.05.09 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
WANG, Yajun;MORENO, George, T.;ZHANG, Boyan;ZHANG, Liangyi;FARNAN, Dell;PATAPOFF, Tom |
分类号 |
G01N;C07K |
主分类号 |
G01N |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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