| 摘要 |
PURPOSE:To make highly sensitive reagent for estimation of hedrogen peroxide by using a specific imidazole derivative. CONSTITUTION:An imidazole derivative expressed by the formula is used for a reagent for the analysis of hydrogen peroxide. Using such a reagent, hydrogen peroxide and the imidazole derivative expressed by the formula are made to react with each other, so as to produce dyestuff of 1mol with hydrogen peroxide of 1mol. The analysis is performed by detecting the dyestuff thus produced. This reagent is made applicable in a wider sphere as a reagent for estimation by combining the imidazole derivative expressed by the formula with peroxidase and further with oxidase. In other words, in the case when a material to be quantified finally produces hydrogen peroxide under the effect of oxidase, the combination of the oxidase with a reagent system of the present invention enables the estimation of various subject materials through the estimation of the hydrogen peroxide thus produced. In the formula, R<1> denotes an aryl in which an ortho-position or a para-position is substituted by a hydroxyl-radical. R<2> and R<3> denote an aryl, a substituted aryl or a furyl-radical, respectively, and at least one of R<2> and R<3> denotes a furyl radical. |
