| 摘要 |
PROBLEM TO BE SOLVED: To obtain a biosensor which suppresses an effect due to red corpuscles coexisting with a substrate in blood and by which the substrate can be quantitatively determined with high accuracy by a method wherein an electrode system which contains a working electrode and a counter electrode is formed on an electrical insulating substrate, a hydrophilic polymer layer is formed and a reaction layer containing an electron acceptor which uses an oxidation-reduction enzyme and a sodium salt is formed. SOLUTION: A silver paste is printed, by a screen printing operation, on an electrical insulating substrate 1 composed of polyethylene terephthalate, and lead wires 2, 3 are formed. Then, a conductive carbon paste which contains a resin binder is printed on the substrate 1, and a working electrode 4 is formed. Then, an insulating paster is printed, and an insulating layer 6 is formed. In addition, a conductive carbon paste which contains a resin binder is printed so as to come into contact with the lead wire 3 and a ring-shaped counter electrode 5 is formed. Then, a hydrophilic polymer layer 8 and a reaction layer 7 containing an electron acceptor which uses enzymes and a sodium salt are formed. Sodium ions, which are generated when the sodium salt is dissolved in a sample liquid, act little so as to collapse the shape of red corpuscles in blood. |
