DISSOLUTION-ENHANCED TIME-RESOLVED FLUOROIMMUNOASSAY BASED ON RARE EARTH NANOMATERIAL

摘要

This invention relates to a rare earth nanomaterial labeled biomolecule, its labeling method and a dissolution-enhanced time-resolved fluoroimmunoassay based on the rare earth nanomaterial. The rare earth nanomaterial serves as a label having stable properties, large specific surface area, strong modifiability, low-cost and thousands of lanthanide ions contained in each nanocrystal, the labeling ratio of rare earth ions can be greatly improved. Furthermore, the rare earth nanomaterial can be less affected by exogenous rare earth ions, unaffected by anticoagulants, and has broader applicability; after the immune complex was formed by labeling the biomolecules with the nanomaterial containing rare earth, an enhancer solution was added to allow the rare earth nanomaterial to dissolve into the rare earth ions, which can in turn form new signaling molecules with the chelates in the enhancer solution to induce intramolecular and intermolecular energy transfer, thereby significantly increasing fluorescence intensity by about a million times to greatly enhance the detection sensitivity by using time-resolved fluorescence assay.

主权项

1. A rare earth nanomaterial labeled biomolecule used in time-resolved fluoroimmunoassay, wherein said biomolecule includes biotin, avidin, antibody or aptamer.
Preferably, said rare earth nanomaterial labels said biomolecules via chemical coordination method or physical adsorption method.