LC-MS configuration for purification and detection of analytes having a broad range of hydrophobicites

摘要

Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system. The methods, systems, and kits described herein provide for substantially increased speed/throughput and ease of use for a wide range analytes with essentially no compromise in specificity for individual analytes relative to previously described methods.

主权项

1. A method, comprising:
selecting pre-set system parameters adapted for chromatographic separation and mass spectrometric analysis of analytes in a biological fluid sample and spanning a log partition coefficient (log P) range of about −1.2 to about 6, the system parameters being selected from the group consisting of liquid chromatography system parameters and mass spectrometry system parameters; providing a biological fluid sample comprising at least one analyte having a log P in a range of about −1.2 to about 6; purifying the at least one analyte based on the selected liquid chromatography system parameters using a single analytical liquid chromatography column of a liquid chromatography system, one aqueous mobile phase buffer solution of the liquid chromatography system, and one non-aqueous mobile phase buffer solution of the liquid chromatography system; and analyzing the at least one analyte based on the selected mass spectrometry system parameters using a mass spectrometer.