METHODS AND SYSTEMS FOR CELL STATE QUANTIFICATION

摘要

Systems, methods, libraries, kits, and computer software tools are provided for designing and producing engineered cells. Such engineered cells can be used for cell state quantification, such as genome, transcriptome and/or proteome quantification. In one aspect, an engineered cell having a plurality of artificially designed oligonucleotides introduced into the genome of the cell is provided. The oligonucleotides are each located in proximity of a gene of interest encoding a protein of interest, and are different from one another. The oligonucleotides can each encode a unique peptide tag for each protein of interest, wherein each peptide tag has a unique quantitatively measurable value such as mass-to-charge ratio which can be quantified by a mass spectrometer. The engineered cell is capable of expressing a plurality of proteins of interest each fused to its corresponding unique peptide tag, wherein each peptide tag is capable of being released therefrom.

主权项

1. An engineered cell comprising a plurality of heterologous oligonucleotides introduced therein,
wherein each of the plurality of heterologous oligonucleotides encodes a unique peptide tag for tagging a preselected protein of interest, wherein each unique peptide tag has a different quantitatively measurable value, wherein the engineered cell is designed to express a plurality of proteins of interest, each fused to its corresponding unique peptide tag, and wherein each unique peptide tag is capable of being released from its corresponding preselected proteins of interest via proteolytic cleavage.