MODERATION OF CRUSTACEAN ANDROGENIC GLAND PEPTIDE FOR MONO SEX CULTURE

摘要

This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

主权项

1. A method of producing a monosex culture of crustacean, comprising the steps of
a) obtaining a sperm sac from a male crustacean; b) mixing the sperm sac with a double-stranded RNA (dsRNA) comprising a nucleotide sequence of crustacean insulin-like androgenic gland peptide (IAG), thereby obtaining a mixture of the sperm sac and the dsRNA; c) contacting the mixture obtained from step b) with a female crustacean, wherein the eggs of the female crustacean are fertilized by the sperm sac in the mixture, thereby obtaining a plurality of fertilized eggs; and d) culturing the fertilized eggs obtained from step c) under appropriate conditions to obtain a monosex culture of crustacean having the phenotype of a female crustacean.