主权项 |
1. A population of virus-like particles (VLPs) produced by a method comprising:
(a) providing a population of VLPs that have been expressed by a prokaryote, wherein each of said VLPs comprises a coat polypeptide of a MS2 or PP7 bacteriophage, wherein said coat polypeptide is modified by insertion of an immunogenic heterologous peptide of Nipah Virus within the amino acid sequence of said coat polypeptide at a site corresponding to the A-B loop, wherein said heterologous peptide is displayed on said VLPs and said VLPs optionally encapsidate said bacteriophage mRNA; and (b) wherein said heterologous peptide comprises at least one peptide selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, and SEQ ID NO: 38; and (c) exposing said population of VLPs obtained in step (a) to Nipah Virus-neutralizing antibodies to bind to said VLPs, wherein said bound VLPs are isolated. |