SORTASE-MEDIATED COUPLING OF IMMUNOGENIC POLYSACCHARIDE-PROTEIN CONJUGATES AND THEIR USE

摘要

Disclosed are immunogenic compositions containing a polysaccharide-sortase conjugate. The polysaccharide is an antigen, and the sortase is a carrier protein. The sortase is covalently linked to the polysaccharide antigen by a linker including a sortase recognition sequence. Also disclosed are immunogenic compositions containing a polysaccharide-protein conjugate, the conjugate containing a polysaccharide antigen and a carrier protein. The polysaccharide antigen is covalently linked to the carrier protein by a linker including a sortase recognition sequence and a polyglycine motif present at the N-terminus of the carrier protein. Also disclosed are pharmaceutical compositions containing the immunogenic composition and a pharmaceutically acceptable excipient, methods of making the immunogenic compositions, and methods of generating immune response in a subject using the pharmaceutical composition.