METHODS FOR STAINING CELLS FOR IDENTIFICATION AND STORING

摘要

The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided.

主权项

1. A method for distinguishing sperm based on DNA content, comprising:
staining sperm with a DNA-selective fluorescent dye by missing the sperm with the dye at a temperature substantially equal or less than 39° C.; mixing the sperm with at least one nanoparticle; electroporating the sperm, the dye and the at least one nanoparticle for a period of time to provide substantially uniform staining and concomitantly to substantially preserve sperm viability; exposing the sperm to a light source to cause the stained DNA to fluoresce; detecting a pre-determined fluorescence of the stained DNA, the pre-determined fluorescence corresponding to DNA content; sorting the sperm based on the pre-determined fluorescence; and collecting selected sperm from the sorted sperm.