Supports for cell culture and cell sheet detachment and methods for cell sheet detachment

摘要

The invention creates a support for cell culture and cell sheet detachment which has a substrate, whose surface is coated with a conjugate having a disulfide bond-containing amino acid as a spacer and a biopolymer enhancing cell attachment, migration or differentation. Unexpectedly, after being seeded on the support, the cells grow to form one or more layers of cell sheets and the cell sheets can be easily detached from the support by adding a reductant to cleave the disulfide bond. Accordingly, the invention provides a simple and non-toxic method for detachment of cell sheets.

主权项

1. A support for cell culture and cell sheet detachment, consisting of a biocompatible substrate with carboxyl groups on the surface thereof, wherein the surface is in situ grafted with a conjugate having the formula -L-S—S—R-Q through a peptide bond of —C(═O)NH—, wherein L and R each represent a cysteine residue; —S—S represents a disulfide bond that is formed by oxidation of thiol groups of L and R; and Q is a biopolymer; wherein L links to the biocompatible substrate and wherein the biocompatible substrate is transparent TCPS, PET, PVDF, glass, quartz or quartz glass, and wherein Q of the conjugate is, gamma-polyglutamic acid (γ-PGA), collagen, or hyaluronic acid.