发明名称 |
METHODS FOR EFFICIENT, EXPANSIVE, USER-DEFINED DNA MUTAGENESIS |
摘要 |
The presently disclosed subject matter provides methods for the creation of one or more user-defined mutations that can be located anywhere in a target sequence, such as in a gene. These mutations can comprise single mutations, multiple mutations, or a comprehensive codon mutagenesis library, in which all possible single codon substitutions in a gene are created. These methods can be used on a single-stranded or double-stranded template. |
申请公布号 |
US2017002345(A1) |
申请公布日期 |
2017.01.05 |
申请号 |
US201615255879 |
申请日期 |
2016.09.02 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
FIRNBERG ELAD;OSTERMEIER MARC ALAN |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
BALTIMORE MD US |