P38 MAPK INHIBITOR-CONTAINING MEDIUM AND METHOD FOR OSTEOGENIC DIFFERENTIATION

摘要

Here is provided a novel differentiation protocol, which was experimentally shown to give rise to osteoprogenitor cells in vitro. A novel medium composition containing a p38 MAPK inhibitor was found to promote differentiation of human adipose stem cells (hASCs) to osteoprogenitor cells. The early osteogenic precursor cells may further differentiated to osteoblasts. With the present method, it can be produced cells, expected to contribute to treatment and research of conditions, diseases, pathologies, transplant techniques as well as toxicology and drug development related to the skeletal system.

主权项

1. An osteoinductive differentiation medium for differentiation of human adipose stem cells (hASCs) into osteoprogenitor cells, the medium comprising a p38 MAPK inhibitor at a concentration of 0.1 to less than 10 μM, wherein the p38 MAPK inhibitor is
4-(4-Fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)-1H-imidazole or 1-[2-Methoxy-4-(methylthio)-benzoyl]-4-benzylpiperidine.