| 摘要 |
The present invention relates to a method for preparing aptamers binding to new cells and, specifically, to a method for preparing aptamers, comprising a step of eluting aptamers bound to cells by culturing cells in a chip comprising a single or a plurality of chambers, and treating the same with an elution buffer. Cell SELEX-on-a-chip has been developed by applying a microfluid for eluting specific aptamers without damaging cells. It is shown that SELEX can be effectively carried out in a SELEX-on-a-chip, compared with a chip SELEX and a conventional SELEX. It is shown that the method, which has excellent performance, can be applied to aptamers specific to various cancer cells. |
